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KMID : 0615720120290010115
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Jouranl of Korean Acupuncture and Moxibustion society
2012 Volume.29 No. 1 p.115 ~ p.125
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The Inhibitory Effects of Chrthami Semen Oil Pharmacopuncture (CSOP) on Synovial Membranes in Type 2 Collagen-Induced Arthritis Mice
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Back Song-Ook
Kim Eun-Jung
Hwang Ji-Hoo
Yoon Jong-Hwa
Lee Seung-Deok
Kim Kap-Sung
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Abstract
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Objectives: The purpose of this study is to observe the inhibitory effects of Chrthami semen oil pharmacopuncture(CSOP) on CIA (collagen-induced arthritis) mice.
Materials and Methods: Two types of experiments were conducted: in vitro assay, inhibition of MIF mRNA and TNF-α mRNA expressions in synovial membranes was observed, and in vivo assay, 1 ㎕/㎏ CSOP was injected every day to the left Weizhong (BL40) from day 3 to 21 after induction of CIA, and changes in paw edema, apical surface morphology, neovascularization in synovial membranes, fibrosis, pro-inflammatory cytokines production, Th-1 differentiation, and anti-inflammatory effect were investigated.
Results: 1. In synoviocytes of the CIA mice treated with CSOP, MIF mRNA and TNF-α mRNA expressions were down-regulated in a dose-dependent manner. 2. Paw edema of the CIA mice treated with CSOP was diminished. 3. Tissue injury in the synovial membranes, capillary distribution and fibrosis were reduced in CSOP-treated mice. 4. MIF, TNF-α, IL-6, MMP-9 expressions were repressed in CSOP-treated mice during the experiment to observe the inhibitory effect on cytokines production in early stage RA. 5. IL-12 and CD28 were reduced in CSOP-treated mice during the observation of inhibitory effect on Th1 differentiation. 6. PPAR-γ was increased during the experiment to observe the anti-inflammatory effect of CSOP.
Conslusions: The results may suggest that administration treatment using Chrthami semen oil pharmacopuncture decreases the inflammatory response on an Animal Model with CIA.
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KEYWORD
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Chrthami semen oil, pharmacopuncture, type 2 collagen-Induced arthritis, synovial membrane
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